Detection of single nucleotide polymorphisms of Jak2 gene in multiple myeloma by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1234-1237, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-343312
ABSTRACT
This study was purposed to investigate the practicality of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) for detection of single nucleotide polymorphisms (SNP) on jak2 gene in multiple myeloma (MM) cells. The DNA fragment containing 2 SNPs of jak2 (C428T and C643T) was amplified using PCR and was purified. The purified product was used as the template for primer extension (PEX). The small products of allele-specific reaction were purified, the SNPs on jak2 gene of 5 patients with MM and 5 healthy persons were detected by MALDI-TOF MS. The results showed that the distribution of genotype C428T and C643T was not different between MM patients and healthy persons, both of which are homozygous T/T. In conclusion, the method based on MALDI-TOF MS and PEX technique for detecting SNP in jak2 gene is rapid, accurate and reliable method, and can be used in clinical practice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Case-Control Studies
/
DNA Primers
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Polymorphism, Single Nucleotide
/
Janus Kinase 2
/
Genetics
/
Genotype
/
Methods
/
Multiple Myeloma
Type of study:
Diagnostic study
/
Observational study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2009
Type:
Article
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