Interaction between mixed-lineage leukemia and Menin proteins / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1130-1134, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-343333
ABSTRACT
The study was aimed to investigate the subcellular localization of mixed-lineage leukemia (MLL) and Menin proteins, and to explore the interaction between these two proteins. The recombinant eukaryotic cell expression vectors of pcDNA3.1-myc-MLL and pCMV-flag-Menin were constructed respectively, and transfected into the HEK293T cells. Immunofluorescence technique was used to observe the subcellular localization of the two proteins. Co-immunoprecipitation and Western blot methods were applied to evaluate the expression and interaction of the two proteins. The results showed that MLL and Menin proteins could be co-localized in cell nuclei, and the study of binding in vivo revealed that MLL protein could be detected in the immunoprecipitation complex of anti-FLAG, while Menin proteins could also be found in the immunoprecipitation complex of anti-MYC. It is concluded that MLL and Menin proteins co-localized in cell nuclei have same location and the interaction exists between MLL and Menin proteins.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Leukemia
/
Chromosome Mapping
/
Proto-Oncogene Proteins
/
Homeodomain Proteins
/
Protein Interaction Mapping
/
DNA-Binding Proteins
/
Myeloid-Lymphoid Leukemia Protein
/
HEK293 Cells
/
Genetic Vectors
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2009
Type:
Article
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