Expression of anti-platelet glycoprotein specific antibodies and anti-HLA antibodies in idiopathic thrombocytopenic purpura / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1032-1035, 2009.
Article
in Zh
| WPRIM
| ID: wpr-343356
Responsible library:
WPRO
ABSTRACT
In order to investigate the expression of the anti-platelet glycoprotein specific antibodies and anti-HLA antibodies in idiopathic thrombocytopenic purpura (ITP), 45 patients with ITP were selected in this study. An easy PCR-SSP assay was used to detect single-nucleotide polymorphisms or deletion in HPA and HLA systems. The anti-platelet glycoprotein specific antibodies and anti-HLA antibodies in plasma or platelet eluate were tested with a solid phase ELISA. The results indicated that the anti-platelet glycoprotein specific antibodies were detected in plasma or platelet eluate of 45 patients, among which anti-GPIIb/IIIa/and anti-GpIb/IX were most common. Both the anti-platelet glycoprotein specific antibodies and anti-HLA antibodies were found in plasma of 11 patients. Pedigree investigation in 2 patients (case 37 and case 40) was carried out, the results showed that anti-platelet glycoprotein specific antibodies and anti-HLA antibodies detected in 2 patients closely related to incompatibility with platelet antigens and HLA antigens in parents. In conclusion, the results suggested that detection of the anti-platelet glycoprotein specific antibodies and anti-HLA antibodies in plasma or platelet eluate in combination with investigation of clinical manifestation of patients is important for diagnosis of idiopathic thrombocytopenic purpura.
Full text:
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Index:
WPRIM
Main subject:
Blood
/
Platelet Membrane Glycoproteins
/
Antibodies, Anti-Idiotypic
/
Purpura, Thrombocytopenic, Idiopathic
/
Antigens, Human Platelet
/
Platelet Glycoprotein GPIIb-IIIa Complex
/
Platelet Glycoprotein GPIb-IX Complex
/
Allergy and Immunology
/
HLA Antigens
Limits:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
/
Newborn
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2009
Type:
Article