Inhibitory effects of ginkgolide B on proliferation of bovine aortic smooth muscle cells / 药学学报
Acta Pharmaceutica Sinica
;
(12): 90-93, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-343394
ABSTRACT
<p><b>AIM</b>To investigate the effects of ginkgolide B (GB) on proliferation of bovine aortic smooth muscle cells (SMC) and its related mechanisms.</p><p><b>METHODS</b>After pretreating with GB or the mixture of ginkgolide A and B (GAGB) at 37 degrees C for 0.5 h, the VSMC were treated with or without angiotensin II (Ang II) for 24 h. The proliferation of SMC was evaluated by 3H-thymidine incorporation and the cell cycle phase was measured by flow cytometry. Cytotoxicity was reflected by MTT and lactate dehydrogenase (LDH) activity of the supernatant.</p><p><b>RESULTS</b>Whether or not treated with Ang II, GB and GAGB were shown to suppress SMC proliferation in concentration-dependent fashion at concentrations ranging from 10(-9) mol.L-1 to 10(-5) mol.L-1. The inhibitory effects appeared to be related to a G1-->S block in cell cycle traverse.</p><p><b>CONCLUSION</b>The suppression of SMC proliferation by GB might not only be contributed by blockage of the PAF receptor activity.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aorta
/
Pharmacology
/
DNA
/
Platelet Membrane Glycoproteins
/
Cell Division
/
Cells, Cultured
/
G1 Phase
/
Cell Biology
/
Myocytes, Smooth Muscle
/
Receptors, G-Protein-Coupled
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2002
Type:
Article
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