Experimental study of skin wound healing with epidermal stem cells from human hypertrophic scar in nude mice / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 289-293, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-343439
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of epidermal stem cells from human hypertrophic scar (HS-ESCs) on the skin wound healing in nude mice.</p><p><b>METHODS</b>40 mice were randomly divided into two groups as experimental group (n = 20) and control group (n = 20). Wounds, 1 cm in diameters, were made on every mouse back. The wounds were treated with HS-ESCs and erythromycin ointment in experimental group, or only with erythromycin ointment in control group. The wound healing was observed during the following 14 days. The expression of collagen-I, collagen-III, epidermal growth factor (EGF), fibroblast growth factor (FGF2) , transforming growth factor (TGFbeta1, and TGFbeta2) were studied.</p><p><b>RESULTS</b>The wound healing time in the experimental group was (20.8 +/- 0.84) d, which was (25.6 +/- 0.89) d in the control group. HE staining revealed that the extent of vascularization in the experimental group was 11.60 +/- 0.55, while it was 8.04 +/- 0.33 in the control group. Immunochemistry analysis showed the expression of collagen-I, collagen-III, EGF, FGF2, TGFbeta1, and TGFbeta2 in the experimental group were significantly higher, compared with those in control group (P < 0.05).</p><p><b>CONCLUSION</b>HS-ESCs may promote wound healing through enhancement of the vascularization of the wound tissue and the expression of growth factors.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Skin
/
Stem Cells
/
Wound Healing
/
Wounds and Injuries
/
Cicatrix, Hypertrophic
/
Cell Biology
/
Stem Cell Transplantation
/
Epidermis
/
Mice, Nude
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Plastic Surgery
Year:
2014
Type:
Article
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