Effects of flunarizine on T-type calcium channels in mouse spermatogenic cells / 中华男科学杂志
National Journal of Andrology
;
(12): 594-601, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-343566
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Flunarizine (Flu) on T-type calcium currents (ICaT) in spermatogenic cells.</p><p><b>METHODS</b>Ca2+ currents were obtained in acutely dissociated mouse spermatogenic cells using whole-cell patch clamp technique and the effects of Flu on ICaT were observed.</p><p><b>RESULTS</b>Flu of 0.1, 1, 10, 100 micromol/L inhibited ICaT in mouse spermatogenic cells significantly with the K50 value of 0.289 micromol/L (P < 0.05). With the holding potential at -90 mV and stimulating potential at -30 mV, the inhibition rates of Flu were (23.34 +/- 2.76)%, (46.04 +/- 3.52)%, (62.52 +/- 3.70)% and (73.52 +/- 3.12)%, respectively.</p><p><b>CONCLUSION</b>Flu has significant inhibitory effects on ICaT in mouse spermatogenic cells, with concentration dependence. Ca(v)3.2 is the main contributor to T-type Ca2+ currents in spermatogenic cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Spermatids
/
Calcium Channel Blockers
/
Flunarizine
/
Cells, Cultured
/
Patch-Clamp Techniques
/
Calcium Channels, T-Type
/
Dose-Response Relationship, Drug
/
Mice, Inbred ICR
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2006
Type:
Article
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