Correlation of epididymal protease inhibitor Eppin and Semenogelin on human ejaculated spermatozoa / 中华男科学杂志
National Journal of Andrology
;
(12): 428-434, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-343605
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the correlation of epididymal protease inhibitor(Eppin) and Semenogelin(Sg) on human ejaculated spermatozoa.</p><p><b>METHODS</b>The experimental approaches include (1) Immunoprecipitation of Eppin with anti-Eppin from semen; (2) Colocalization of Eppin and Sg by immunofluorescence; (3) Immunoprecipitation of rEppin and rSg;(4) Far-Western blotting of rEppin and rSg;(5) Competition of saturated 125I-rSg binding to rEppin with unlabeled Sg, and direct binding of 125I-rSg to rEppin on a blot; (6) Autoradiography of 125I-rSg with rEppin.</p><p><b>RESULTS</b>Eppin-Sg complex present on the surface of human ejaculated spermatozoa, Cys-239 is the only cystein for rEppin binding rSg. Reduction and carboxymethylation of Cys-239 blocks binding of 125I-rEppin to rSg.</p><p><b>CONCLUSION</b>Our study demonstrates that Eppin and Sg bind to each other on human ejaculated spermatozoa. A disulfide linkage occurs between Sg and Eppin, indicating the specificity of binding.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Binding
/
Spermatozoa
/
Recombinant Proteins
/
Proteins
/
Chemistry
/
Seminal Vesicle Secretory Proteins
/
Proteinase Inhibitory Proteins, Secretory
/
Metabolism
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2006
Type:
Article
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