Establishment of the control substance of plant drug and fingerprints of Coptis chinensis / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 622-627, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-343795
ABSTRACT
<p><b>OBJECTIVE</b>To establish the control substance of plant drug (CSPD) of Coptis chinensis Franch. and its proton nuclear magnetic resonance (1H NMR) and high performance liquid chromatography (HPLC) fingerprints for the purpose of original identification.</p><p><b>METHODS</b>The CSPD and their 1H NMR and HPLC fingerprints of Coptis chinensis were obtained by standardized procedure. Chemical components were isolated from the CSPD by silica gel column chromatography. By elucidation of their structures, the assignments of the characteristic signals in fingerprints could be achieved. RESULTS; The 1H NMR and HPLC fingerprints of the samples from various sources had wonderful reproducibility and characteristic features. Furthermore, five main compounds were isolated from CSPD and their structures were authenticated by spectral analysis as palmatine chloride, berberine chloride, epiberberine chloride, coptisine chloride, and jatrorrhizine chloride, respectively. The 1H NMR and HPLC fingerprints of the CSPD of Coptis chinensis showed mainly the characteristic signals of the berberine-type compounds isolated in this work.</p><p><b>CONCLUSION</b>The 1H NMR and HPLC fingerprints of the CSPD of Coptis chinensis exhibit the structures and total composition of the main active constituents in it, and can be used for its original identification and quality evaluation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Quality Control
/
Berberine
/
Berberine Alkaloids
/
Magnetic Resonance Spectroscopy
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Coptis
Type of study:
Prognostic study
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2004
Type:
Article
Similar
MEDLINE
...
LILACS
LIS