Determination of ABO blood group genotypes with one tube PCR reaction / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1117-1119, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-343813
ABSTRACT
This study was aimed to establish one tube PCR reaction technique to determine ABO blood group genotypes. Salting-out method was adopted to extract genomic DNA; one tube polymerase chain reaction with GeneScan technique was used to identify ABO genotypes. The results showed that the ABO genotypes of 132 samples were in accordance with the phenotypes determined by serological technique. The frequencies of A, B and O were 0.205, 0.159 and 0.636 respectively. AA, AO, AB, BB, BO and OO genotypes were 8 (6.1%), 31 (23.5%), 7 (5.3%), 6 (4.5%), 23 (17.4%), and 57 (43.2%) respectively. It is concluded that one tube polymerase chain reaction with GeneScan technique can determine the genotypes of ABO blood group.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
ABO Blood-Group System
/
Polymerase Chain Reaction
/
Reproducibility of Results
/
Gene Frequency
/
Genetics
/
Genotype
/
Methods
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article
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