Peripheral stem cell mobilization with medium dose of G-CSF in normal donors / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1038-1040, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-343832
ABSTRACT
The study was aimed to investigate the mobilization effect of medium dose of granulocyte colony stimulating factor (G-CSF) in allogeneic peripheral stem cell transplantation and changes of T lymphocyte subgroup in PBMNC before and after mobilization. G-CSF was administered at 600 microg/d (i.e. 300 microg i.v. twice a day) for successive 5 days to 31 matched sibling or unrelated donors for the mobilization. Stem cells were harvested on the fourth day. FACS was used to analyze the NC, MNC and T lymphocyte subgroups. The results showed that the number of NC, MNC, CD34(+) cells and CFU-GM in dose of 600microg/d significantly increased (P < 0.05), compared with 300 microg/d; the time for hematological reconstruction was significantly shortened (P < 0.05); the ratio of adverse effects was not obviously increased (P > 0.05) and the median percentage of CD3(+) lymphocytes before mobilization was 46.96% [(32.36-57.45)%], but 40.94% [(25.31-48.9)%] after mobilization, while the ratio of CD4(+)/CD8(+) did not significantly changed. It is concluded that the administration of G-CSF 600 microg/d in allo-PBSCT has a good effect in the mobilization of PBSC with minor side effects, which can markedly promote hematopoietic reconstitution after transplantation. The relative amount of CD3(+) lymphocytes significantly decreased and the ratio of CD4(+)/CD8(+) remained unchanged, which may lead to alleviation of a GVHD after PBSCT.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Blood Donors
/
Recombinant Proteins
/
Granulocyte Colony-Stimulating Factor
/
T-Lymphocyte Subsets
/
Antigens, CD34
/
Hematopoietic Stem Cell Mobilization
/
Cell Biology
/
Peripheral Blood Stem Cell Transplantation
/
Allergy and Immunology
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article
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