Mechanism of differentiation and apoptosis in leukemia cells induced by tributyrin / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 774-777, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-343889
ABSTRACT
To elucidate the possible mechanism of differentiation and/or apoptosis in NB4, K562 cells induced by tributyrin (TB), a histone deacetylase inhibitor (HDACi), the level of acetylated histone H3 was detected by Western blot, p21(WAF1) expression was detected by semi-quantitative RT-PCR. The results showed that histone H3 hyperacetylation was detected in NB4 (or K562) cells after treatment with TB 0.1 mmol/L (or TB 0.5 mmol/L) for 16 hours in a dose-dependent manner. p21(WAF1) dose-dependently increased at RNA level in these two cell lines treated by TB 0.1 mmol/L. The level of p21(WAF1) mRNA increased at 2 hours after TB treatment, reaching peak at 16 hours, and maintaining for 48 hours. In conclusion, the mechanism of differentiation and apoptosis in NB4, K562 cells induced by tributyrin may associate with its up-regulation of acetylated histone and p21(WAF1) mRNA level.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Acetylation
/
Triglycerides
/
RNA, Messenger
/
Histones
/
Leukemia
/
Gene Expression Regulation, Neoplastic
/
Cell Differentiation
/
Blotting, Western
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS