Arsenic trioxide induced leukemic cell apoptosis relative to NF-kappaB activation / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 764-768, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-343891
ABSTRACT
To investigate the relationship of As(2)O(3)-induced leukemic cell apoptosis with NF-kappaB activation and expression of VEGF, MMP9, apoptosis of K562-n cells induced by As(2)O(3) was analyzed by Annexin V, the dynamic changes of NF-kappaB, MMP9 and VEGF expressions were detected by immunohistochemistry. The results showed that activity of NF-kappaB could be increased, accompanied by higher level of expression of MMP9 and VEGF when apoptosis of K562-n cells was induced by As(2)O(3). Dexamethasome not only increased significantly the apoptotic rate, but also suppressed the activation of NF-kappaB of K562-n cells induced by As(2)O(3). Furthermore, there was a positive correlation between the expression of MMP9, VEGF and the activity of NF-kappaB. It is concluded that As(2)O(3) can induce apoptosis, in the meanwhile, activate NF-kappaB and up-regulate expression of MMP9 and VEGF in K562-n cell line. The mechanism of apoptosis of K562-n cells enhanced by dexamethasome may be related to suppression of the activation of NF-kappaB and expression of MMP9 and VEGF.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxides
/
Pharmacology
/
Arsenicals
/
Immunohistochemistry
/
NF-kappa B
/
Apoptosis
/
K562 Cells
/
Matrix Metalloproteinase 9
/
Vascular Endothelial Growth Factor A
/
Flow Cytometry
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article
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