Study on the expansion of megakaryocyte progenitors in vitro from cord blood / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 660-663, 2005.
Article
in Zh
| WPRIM
| ID: wpr-343910
Responsible library:
WPRO
ABSTRACT
This study was aimed to investigate the effect of various cytokines on megakeryocytes expansion in vitro from human cord blood CD34(+) cells in order to establish an optimal culture system for MK expansion. Mononuclear cells were obtained by Ficoll-Hapaque density gradient separation. CD34(+) cells were positively isolated using a CD34 progenitor cell isolation kit. CD34(+) cells were placed into 24 well plates at a concentration of 2 x 10(4) per well. Each well contained 1 ml of IMDM with the present of effective MK cells growth cytokines. Clonogenic potentials of MK progenitor were assayed using a methylcellulose cultures system. The results suggested that four cytokines (IL-3 + IL-6 + TPO + FLT3L) culture system could effectively induce and expand cord blood CD41(+) MK cells. The number of CD41(+) cells expanded 154.67 +/- 32.21-fold on day 7, and 193.23 +/- 25.24-fold on day 14. In conclusion, established expansion system in vitro for MK cells provides experimental foundation for recovery of platelets after cord blood transplantation.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Stem Cells
/
Thrombopoietin
/
Blood Platelets
/
Megakaryocytes
/
Cell Differentiation
/
Cells, Cultured
/
Interleukin-6
/
Interleukin-3
/
Antigens, CD34
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article