Effect of guilu erxianjiao in suppressing splenic T-lymphocyte apoptosis in mice undergoing chemotherpy / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 339-342, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-344007
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Guilu Erxianjiao (GLEXJ) in suppressing chemotherapy induced cell apoptosis in mice and to discuss its possible acting mechanism.</p><p><b>METHODS</b>Seventy-two tumor-bearing Kunming mice were randomly divided into 6 groups, Group A, treated with normal saline; Group B treated with cyclophosphamide (CTX), Group C treated with Chinese herbal medicine, and Group D-F, the three combined treated group received CTX plus high, moderate and low dose GLEXJ. All mice were sacrificed after 9-day medication, their splenic tissues were taken for determining T-lymphocyte apoptosis with flow cytometer, the expression of bcl-2 mRNA and Caspase-3mRNA detected by RT-PCR.</p><p><b>RESULTS</b>As compared with Group A, FITC+/PI- cell proportion of spleen T-lymphocyte cells and Caspase-3mRNA expression were higher, while bcl-2 mRNA expression was lower in Group B; as compared with Group B, the former two indexes were lower and the latter was higher in the three combined treated groups (all P <0.05).</p><p><b>CONCLUSION</b>GLEXJ could efficiently suppress the splenic T-lymphocyte apoptosis in tumor bearing mice undergoing chemotherapy, one of its mechanisms may be through up-regulating of bcl-2 mRNA expression and down-regulating of Caspase-3mRNA expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spleen
/
Drugs, Chinese Herbal
/
T-Lymphocytes
/
Random Allocation
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
Cell Biology
/
Cell Proliferation
/
Drug Therapy
/
Caspase 3
Type of study:
Controlled clinical trial
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2008
Type:
Article
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