Effects of hyperin on the cccDNA of duck hepatitis B virus and its immunological regulation / 药学学报
Acta Pharmaceutica Sinica
; (12): 1440-1444, 2009.
Article
in Zh
| WPRIM
| ID: wpr-344057
Responsible library:
WPRO
ABSTRACT
The aim of this study is to investigate the effect of hyperin on the cccDNA of duck hepatitis B virus and its immunological regulation. Duck hepatitis B virus (DHBV) infection model and normal mouse spleen lymphocyte were used to evaluate the anti-HBV and immunoregulation effects. The DHBV-DNA of serum was detected at different time points by using serum DOT-BLOT hybridization. Polymerase chain reaction (PCR) was used for the determination of nuclear covalent closed circular DNA (cccDNA). Cytokine secretion was determined by ELISA method. DHBV-DNA were inhibited by hyperin (25 or 50 mg x kg(-1)), while cccDNA of liver could be eliminated efficiently by hyperin (25 or 50 mg x kg(-1), P < 0.05, P < 0.01). The T helper 1 effector cytokine was markedly enhanced by hyperin (25 or 50 microg x mL(-1), P < 0.01). In conclusion, hyperin has anti-HBV activity via multiple targets and pathways, and cccDNA may be one of the important targets.
Full text:
1
Index:
WPRIM
Main subject:
Antiviral Agents
/
Pathology
/
Pharmacology
/
Quercetin
/
Spleen
/
Virology
/
DNA, Circular
/
DNA, Viral
/
Lymphocytes
/
Hepatitis B Virus, Duck
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2009
Type:
Article