Preparation of brain targeted immunoliposomes / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1285-1290, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-344084
ABSTRACT
To prepare a kind of effective non-viral transduction vector, which can deliver exogenous gene into the brain, this vector can be injected through vein system and has the ability to penetrate blood brain barrier. Several groups of materials proportion, type of oil phase, water-oil ratio, phosphatides-cholesterol ratio, temperature of steaming, ultrasonic temperature and time were compared for optimization. Well-constructed immunoliposomes encapsuling LacZ gene were infused into rats through tail vein. 48 h after injection, expression product beta-galactosidase of LacZ gene was detected by histochemistry staining to convince the validity of immunoliposomes as non-viral vectors. The best proportion of synthesis immunoliposomes is as following phosphatides-cholesterol ratio is 11, lipids/drug is 1001, the type of oil phrase is dichloromethane, oil-water ratio is 41, temperature of steaming is 30 degrees C, ultrasonic temperature and time is 10 degrees C and 5 min. At last, 10% trehalose was added as a stabilizer. The entrapment rate is 87.24% and antibody coupling rate is 69%. When immunoliposomes were infused into rats, the expression of LacZ gene could be observed in the brain and periphery organs. Through the best proportion of materials, gene delivering immunoliposomes had been synthesized successfully. This non-viral vector can deliver exogenous gene penetrating blood brain barrier and express in the brain, and will be well-used in the field of gene therapy of cerebral diseases.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Particle Size
/
Plasmids
/
Polyethylene Glycols
/
Receptors, Transferrin
/
Brain
/
Pharmacokinetics
/
Blood-Brain Barrier
/
Tissue Distribution
/
Drug Delivery Systems
/
Beta-Galactosidase
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS