Paeonol attenuates oxygen-glucose deprivation injury and inhibits NMDA receptor activation of cultured rat hippocampal neurons / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1228-1232, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-344089
ABSTRACT
The purpose of this study is to determine if paeonol can protect hippocampal neurons against injury due to oxygen-glucose deprivation (OGD) injury. The rat neurons were cultured in an OGD environment and the model of OGD injury was established. Paeonol and MK-801, a positive control drug, were added before deprivation. Neuron viability was measured by the reduction of MTT; glutamate was analyzed by amino acid analyzer; binding activity of NMDA receptor was evaluated by liquid scintillation counting and the expression of NMDA receptor NR1 subunit mRNA was semiquantitatively determined by RT-PCR. Compared with OGD injury group, paeonol treatment obviously increased cell survival rate and reduced the binding activity of NMDA receptors and the release of glutamate; and down-regulating the expression of NR1 subunit. These results suggest that paeonol may exhibit its protective effect against OGD injury by the action on NMDA receptor of rats.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Acetophenones
/
Pharmacology
/
Plants, Medicinal
/
Protein Binding
/
RNA, Messenger
/
Random Allocation
/
Cell Hypoxia
/
Cell Survival
/
Cells, Cultured
/
Chemistry
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2009
Type:
Article
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