Establishment of rabbit model of juvenile nonalcoholic steatohepatitis / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 240-244, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-344343
ABSTRACT
<p><b>OBJECTIVE</b>To establish a rabbit model of juvenile nonalcoholic steatohepatitis (NASH) for further study.</p><p><b>METHODS</b>Twenty-eight New Zealand rabbit pups were fed with a high-fat diet (standard diet+10 % lard+2 % cholesterol) for 8 or 12 weeks as the two model groups, and 10 rabbits were fed with standard diet as the controls. Liver tissue samples were collected for Heamatoxylin-Eosin staining and pathological examination.</p><p><b>RESULT</b>Typical histological hepatic lesions of NASH were observed in both model groups. Compared with control group, model groups showed a significant increase in serum ALT, AST, TG, TC levels (P <0.01), and decrease in serum adiponectin, IL-10 levels (P <0.05), meanwhile there was no significant difference between two model groups. TC and the degree of liver fatty infiltration were independent determinants of serum adiponectin level by stepwise multiple regression, beta=-1.33, P=0.006 and beta=-0.97, P=0.038, respectively, R square equal to 0.294.</p><p><b>CONCLUSION</b>The juvenile steatohepatitis rabbit model has been established and the level of adiponectin can partly reflect the severity of liver steatosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Blood
/
Dietary Fats
/
Random Allocation
/
Interleukin-10
/
Disease Models, Animal
/
Adiponectin
/
Fatty Liver
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2008
Type:
Article
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