Expression of telomere binding factor 2 (TRF2) on leukemia cell lines and primary leukemia cells / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To detect the expression levels of telomere binding factor 2 (TRF2) on leukemia cell lines and primary leukemia cells.</p><p><b>METHODS</b>The expression of TRF2 mRNA was detected with quantitative real-time RT-PCR in leukemia cell lines and primary leukemia cells. The Western blot analysis was used for the detection of TRF2 protein expression.</p><p><b>RESULT</b>TRF2 was overexpressed in T-cell leukemia cell lines but not in myelogenous leukemia cell lines. Significant higher expression levels of TRF2 were observed in primary leukemia cells from patients with M0 and M1 subtypes of acute myelogenous leukemia (AML) compared with normal control and other subtypes of AML.</p><p><b>CONCLUSION</b>Increased TRF2 expression levels are found in T-cell leukemia cell lines and AML patients with poor prognosis, which suggests that TRF2 expression might be related to the prognosis of leukemia.</p>