Inhomogeneous expression of fusion protein HSA/IL1ra in Pichia pastoris / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 134-138, 2008.
Article
in Zh
| WPRIM
| ID: wpr-344363
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the causes and influencing factors of heterogeneity of HSA/IL1ra fusion protein expression in Pichia pastoris.</p><p><b>METHODS</b>The heterogeneity of HSA/IL1ra fusion protein expressed in Pichia pastoris was studied by removing glycosylation and inhibiting glycosylation, as well as by different ways of fusion, different clones, and different expression host.</p><p><b>RESULT</b>Glycosylation caused expression heterogeneity of fusion protein, but in SMD1168 and some GS115 clones there was no this phenomenon.</p><p><b>CONCLUSION</b>The expression heterogeneity of HSA/IL1ra fusion protein in Pichia pastoris is due to the glycosylation, and different ways of fusion, different clones, different expression host also have some impact.</p>
Full text:
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Index:
WPRIM
Main subject:
Pichia
/
Recombinant Fusion Proteins
/
Serum Albumin
/
Genetic Heterogeneity
/
Escherichia coli
/
Interleukin 1 Receptor Antagonist Protein
/
Genetic Vectors
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Zh
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2008
Type:
Article