Bactericidal activity of GLL-37, a novel derivative of the human antimicrobial peptide LL-37 / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 73-77, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-344374
ABSTRACT
<p><b>OBJECTIVE</b>To develop and investigate GLL-37, a substitution analogue of the human antimicrobial peptide LL-37 with anti-enzymatic degradation activity and improved efficacy.</p><p><b>METHODS</b>The bactericidal activities of LL-37 and newly developed GLL-37 against 6 Gram-negative and -positive bacteria were determined by Broth microdilution assays. The minimum inhibitory concentrations of LL-37 and GLL-37 against E.coli ATCC 25922 in different NaCl concentration medium were also detected. Both peptides were co-incubated with elastase, and then analyzed by PAGE electrophoresis and bactericidal activity determination.</p><p><b>RESULT</b>GLL-37 showed a stronger elastase resistance ability than LL-37, and was significantly more effective than LL-37 under high-salt condition.</p><p><b>CONCLUSION</b>The antimicrobial peptide GLL-37 derived form LL-37 has the potential as a new therapeutic agent for bacterial infections.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Pseudomonas Infections
/
Blood Bactericidal Activity
/
Monocytes
/
Cell Membrane Permeability
/
Antimicrobial Cationic Peptides
/
Therapeutic Uses
/
Drug Therapy
/
Escherichia coli
/
Cathelicidins
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS