Surface markers and functions of human dendritic cells exposed to mobile phone 1800 MHz electromagnetic fields / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 29-33, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-344381
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of mobile phone 1800 MHz electromagnetic fields (EMF) on the surface markers and the functions of human dendritic cells (DC).</p><p><b>METHODS</b>Human DCs were exposed to intermittent 5 min on/10 min off EMF with specific absorption rates (SAR) 4 W/kg for 0 h, 1 h, 12 h or 24 h, respectively. FACS analysis was used to detect the positive percentage of DC surface markers including HLA-DR and co-stimulatory molecules such as CD80, CD86, CD40 and CD11c. CCK-8 kit was adopted to examine the function of allo-mixed lymphocyte reaction (allo-MLR) of DC, and enzyme linked immunosorbent assay (ELISA) to identify the levels of IL-12p70 and TNF-alpha secreted by DC.</p><p><b>RESULT</b>Compared with the sham radiation group, after exposure to the electromagnetic fields for 1 h, 12 h, or 24 h, HLA-DR, CD80,CD86 and CD40 were all declined except CD11c. The ability of DC allo-MLR in each exposure group was decreased significantly (P<0.05), especially in the 24 h exposure group. However, the secreted levels of IL-12p70 and TNF-alpha of DC in each exposure group remained no changed.</p><p><b>CONCLUSION</b>The study showed that EMF exposure could down-regulate the surface molecules and stimulation ability of human DC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Radiation Effects
/
Dendritic Cells
/
Biomarkers
/
HLA-DR Antigens
/
Cells, Cultured
/
B7-1 Antigen
/
Interleukin-12
/
Dendrites
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2008
Type:
Article
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