Dendritic cells pulsed with glioma RNA induce immunity against intracranial gliomas / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 592-597, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-344393
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-tumor effect of dendritic cells (DC) pulsed with G422 glioblastomas RNA in mice bearing intracranially G422 glioblastomas.</p><p><b>METHODS</b>DCs were pulsed in vitro with glioblastomas G422 cell RNA. The tumor-bearing mice were injected intratumorally or subcutaneously with pulsed DCs, PBS, non-pulsed DCs. The survival duration of mice was recorded. Serum levels of cytokine IFN-gamma, IL-2, IL-10, IL-4 were detected. Pathological examination was performed.</p><p><b>RESULTS</b>The survival duration of mice with DC-based vaccine increased significantly(P<0.01). The serum IFN-gamma level was increased (P<0.01) and IL-10 level was decreased (P<0.05) after treatment. Pathological examination showed necrotic tumor in the treatment mice.</p><p><b>CONCLUSION</b>DC vaccination can significantly increase survival duration of mice with intratumoral or subcutaneous administration of vaccines.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
Transplantation
/
Dendritic Cells
/
Brain Neoplasms
/
RNA, Neoplasm
/
T-Lymphocytes, Cytotoxic
/
Random Allocation
/
Glioblastoma
/
Cancer Vaccines
/
Allergy and Immunology
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2007
Type:
Article
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