Heat shock protein 70-HBsAg complex inducing antigen-specific cytotoxic T lymphocyte immune response / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 271-274, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-344426
ABSTRACT
<p><b>OBJECTIVE</b>To explore the possibility of cell-medicated immune response induced with heat shock protein 70 (HSP70)-HBsAg protein complex in vitro.</p><p><b>METHODS</b>HSP70-HBsAg complex was reconstituted in vitro which was injected into mice in order to observe that whether HSP70-HBsAg would stimulate humoral and cellular immune responses. HSP70, HSP70-HBsAg complex and HBsAg were used to activate the dentritic cell (DC) individually, which would initiate homogeneic T lymphocyte to transform to cytotoxic T lymphocyte (CTL). The cytotoxicity of CTL was detected with MTT assay.</p><p><b>RESULTS</b>HSP70-HBsAg complex elicited both humoral and cellular immune responses against HBsAg in mice. Specific CD8+ CTL response was readily induced by HSP70-HBsAg complex and HBsAg, especially the former.</p><p><b>CONCLUSIONS</b>HSP70-HBsAg complex is immunogenic and HSP70 can lead to great efficient CTL response. And HSP70-HBsAg complex may be used as a protein vaccine for immunotherapy for chronic hepatitis B.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Dendritic Cells
/
T-Lymphocytes, Cytotoxic
/
Cells, Cultured
/
Chemistry
/
Immunization
/
HSP70 Heat-Shock Proteins
/
Bodily Secretions
/
Allergy and Immunology
/
Genetics
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2003
Type:
Article
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