Nuclear factor kappa B activity and cell viability of SMMC-7721 inhibited by mutated inhibitor kappa B alpha / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 222-224, 2003.
Article
in Zh
| WPRIM
| ID: wpr-344447
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibition consequence of NF-kappaB activity and cell viability by transfecting mutated inhibitor kappa B alpha (mI(kappa)B(alpha)) into liver cancer cell line of SMMC-7721 cells.</p><p><b>METHODS</b>The nucleic proteins of SMMC-7721 cells transfected with mI(kappa)B(alpha) plasmid and cells with empty pcDNA3 vector were used to determine not only the binding of the 32P-labelled kappaB probes by EMSA, but also the expression of NF-kappaB by western blot. Cell viability was also analyzed.</p><p><b>RESULTS</b>NF-kappaB nuclear translocation was inhibited remarkably in SMMC-7721 cells transfected with mI(kappa)B(alpha) at 0, 24, 48 and 96 hours. Furthermore, NF-kappaB was not detected in the nucleic protein of mI(kappa)B(alpha) -transfected cells at the same intended time by western blot. Compared with that of control cells, the growth of SMMC-7721 cells transfected with mI(kappa)B(alpha) was suppressed evidently, especially on the second day, the cpm values of mI(kappa)B(alpha) -transfected cells, pcDNA3-transfected cells, and control cells were 5,092.63+/-541.41, 7,851.87+/-72.76, and 8,240.8+/-603.26 respectively (t = 14.29, P<0.01; t = 10.99, P<0.01).</p><p><b>CONCLUSION</b>Stable expression of mI(kappa)B(alpha) in SMMC-7721 cells transfected with mI(kappa)B(alpha) plasmid inhibits NF-kappaB nuclear translocation, then suppresses the cell growth.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Physiology
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Translocation, Genetic
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Transfection
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Cell Division
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NF-kappa B
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Carcinoma, Hepatocellular
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I-kappa B Proteins
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Cell Line, Tumor
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NF-KappaB Inhibitor alpha
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hepatology
Year:
2003
Type:
Article