Preliminary study on hepatitis B virus quasispecies in a patient with chronic hepatitis B / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 219-221, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-344448
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether the hepatitis B virus (HBV) has quasispecies character by studying nucleotide sequence polymorphism and mutation features of HBV PreC/C gene region, and preliminaryly explore the heterogeneity of HBV quasispecies.</p><p><b>METHODS</b>The serum sample was obtained from a patient with chronic hepatitis B, and the whole HBV PreC/C gene region was amplified by PCR and cloned. Thirty-four clones that contained HBV PreC/C gene fragments were sequenced.</p><p><b>RESULTS</b>There were 28 kinds of different nucleotide sequences in 34 clones, and the nucleotide sequences diversity ranged from 0.2% to 2.1%. The mutation points were almost distributed in the whole region, but there wasn't mutation at PreC region nt.1 896 point in all sequences.</p><p><b>CONCLUSION</b>Hepatitis B virus has complex quasispecies character in the patients with chronic hepatitis B.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Hepatitis B virus
/
Promoter Regions, Genetic
/
Classification
/
Sequence Analysis, DNA
/
Hepatitis B, Chronic
/
Allergy and Immunology
/
Genetics
/
Hepatitis B Core Antigens
/
Mutation
Limits:
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2003
Type:
Article
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