Reciprocal priming-boosting role of HBsAg and DNA vaccines / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 212-214, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-344451
ABSTRACT
<p><b>OBJECTIVE</b>To evoke more effective humoral and cell-mediated immunization against hepatitis B virus (HBV) infection.</p><p><b>METHODS</b>HBsAg-primed mice were boosted with HBs-DNA vaccine, and HBs-DNA-primed mice were boosted with HBsAg vaccine. Anti-HBs level was assayed by ELISA and cytotoxic T lymphocyte (CTL) response was tested by lactic acid dehydrogenase (LDH) releasing method two weeks after the boosted immunization.</p><p><b>RESULTS</b>Anti-HBs level and CTL responsive rate at the effector/target cell ratio of 1001 were 0.38 and 36% in HBsAg/HBs-DNA vaccination group, 0.32 and 27% in HBs-DNA/HBsAg vaccination group, 0.48 and 1.5% in HBsAg/HBsAg vaccination group, 0.24 and 68% in HBs-DNA/HBs-DNA vaccination group, respectively.</p><p><b>CONCLUSION</b>Priming with HBs-DNA vaccine followed by boosting with conventional HBsAg vaccine results in greater antibody response (F = 21.19, P < 0.05), and CTL response after HBsAg vaccination can be improved by boosting with HBs-DNA vaccine (F = 165.59, P < 0.05). It brings to better efficacy by combining HBsAg vaccine with HBs-DNA vaccine.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Hepatitis B virus
/
Hepatitis B Vaccines
/
Vaccines, DNA
/
Allergy and Immunology
/
Genetics
/
Hepatitis B
/
Hepatitis B Antibodies
/
Hepatitis B Surface Antigens
/
Immunity, Cellular
/
Antibody Formation
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2003
Type:
Article
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