Hairy root culture optimization and aucubin medical composition production of Eucommia ulmoides / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To introduce the hairy root of Eucommia ulmoides, and screen put the excellent roots.</p><p><b>METHOD</b>Hairy roots were obtained by infecting the different explants of E. ulmoides. Aucubin content was detected by HPLC.</p><p><b>RESULT</b>The best transformation condition was embryonic axis infected by twenty minutes bacillus water dip-dye and three-days co-culture. Liquid culture was better than solid culture, and advantageous to accumulating of biomass and medical contents of hairy root. The E1 was the excellent roots.</p><p><b>CONCLUSION</b>Hairy root of E. ulmoides will be useful for the production of active components in aucubin.</p>