Conditions for extraction of ergosterol from fruits of Ganoderma lucidum using supercritical fluid extration / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the feasibility of supercritical fluid extraction (SFE) for extraction of ergosterols from the fruits of Ganoderma lucidum.</p><p><b>METHOD</b>Optimum extraction conditions were studied by orthogonal tests, the ergosterol were analyzed by RP-HPLC.</p><p><b>RESULT</b>The optimal extraction conditions were pressure as 20 MPa, temperature at 45 degrees C, using ethanol as modifier carrier at the volume of 1 mL x g(-1), and 1.5 hour of extraction time.</p><p><b>CONCLUSION</b>SFE has the superiority of adjustable polarity, and has the ability of extracting ergosterol from fruits of Ganoderma lucidum.</p>