Effects of naringin on proliferation, differentiation and matrix mineralization of MC3T3-E1 cells / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of naringin on the proliferation, differentiation and matrix mineralization of MC3T3-E1 cells in vitro.</p><p><b>METHOD</b>MC3T3-E1 cell lines were taken in vitro model. CCk-8 method was used to observe the proliferation of MC3T3 cells. Lactic acid dehydrogenase cytotoxicity (LDH) test was used to observe the cell toxicity. Bone morphogenetic protein-2 (BMP-2), alkaline phosphatase (ALP) and osteocalcin (OC) were used to observe the cell differentiation. Von kossa calcification staining method was used to observe the cell calcification.</p><p><b>RESULTS</b>The high dosages of the naringin could promote the proliferation of MC3T3-E1 cells at both 12 h and 24 h. While, low dosages did not show the same capability. LDH test showed that the cytotoxicity percentages in all six naringin treated groups were quite low. BMP-2 cytoimmunochemistry test showed that the three naringin treated group (10, 1, 0.1 micromol x L(-1)) showed higher brown coloration in cytoplasm than the control group at both 24 h and 48 h. 1, 0.1 micromol x L(-1) naringin raised ALP activity of MC3T3-E1 cells at 48 h (P < 0.05). Meanwhile, 0.1 micromol x L(-1) naringin increased the ALP activity at 72 h (P < 0.05). 10 and 1 micromol x L(-1) naringin increased the capability of MC3T3-E1 cell to synthesize osteocalcin during 8th - 12th dsince adding the medicine (P < 0.05). Naringin did not show the positive effects on cell calcification.</p><p><b>CONCLUSIONS</b>Naringin could promote proliferation and differentiation of MC3T3-E1 cells.</p>