Effects of recombinant human osteoprotegerin and recombinant RANK protein on the differentiation of osteoclast precursors / 中国骨伤

ABSTRACT

<p><b>OBJECTIVE</b>To compare the effect of recombinant OPG-Fc and recombinant RANK protein on the differentiation of osteoclast precursors.</p><p><b>METHODS</b>Mouse osteoblasts cell lines were incubated with osteoclast precursors cell lines RAW 264.7 for 9 days with 10(-5) g/L rhRANK or rhOPG-Fc or PBS added to the coculture system. TRAP stain positive cells counting and cortical bone pit formation counting were performed in the 9th day.</p><p><b>RESULTS</b>Multinuleated TRAP stain positive cells were observed in the cocluture systems after 6 days incubation,and plenty of mature osteoclasts could be observed in the 9th day. With the addition of 10(-5) g/L rhOPG-Fc or rhRANK, multinucleated giant cells and cortical bone pit formation couting decreased significantly compared with the control group, and the rhRANK group decreased more significantly than the rhOPG-Fc group.</p><p><b>CONCLUSIONS</b>Both rhOPG-Fc and rhRANK can inhabit the differentiation of osteoclast precursors and prevent them forming mature osteoclasts,moreover,the rhRANK shows the significant inhabition effect than the rhOPG-Fc.</p>