Effects of geniposide on SNP-induced apoptosis of chondrocyte and cell cycle / 中国骨伤
China Journal of Orthopaedics and Traumatology
;
(12): 232-235, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-344752
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of Geniposide on SNP(sodium nitroprusside)-induced apoptosis of chondrocyte in vitro and cell cycle.</p><p><b>METHODS</b>The chondrocyte of three-week-old SD rats were separated and cultivated. The second generation of chondrocyte cells were involved in experiment. Chondrocyte proliferation was measured by assay; flow cytometer were adopted to observe cell cycle and apoptosis rate; NO examination adopted nitrate reductase method.</p><p><b>RESULTS</b>Geniposide could significantly decrease the percentage of SNP-induced chondrocytes in G0/G1 phase and increased percentage in S phase and G2/M phase. The apoptosis of chondrocyte and the concentration of NO in the culture supernatants was reduced significantly (r=0.917, P<0.01).</p><p><b>CONCLUSION</b>Geniposide could impact SNP-induced apoptosis of chondrocyte by reducing the concentration of NO in the culture supernatants, promoting proliferation of chondrocytes, which is a probable and important mechanism of Geniposide preventing osteoarthritis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoarthritis
/
Pharmacology
/
Physiology
/
Nitroprusside
/
Cell Cycle
/
Rats, Sprague-Dawley
/
Apoptosis
/
Chondrocytes
/
Iridoids
/
Therapeutic Uses
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Orthopaedics and Traumatology
Year:
2013
Type:
Article
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