Clinical application and analysis of hepatitis C virus NS3 antigen detection by ELISA in human serum / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 294-299, 2007.
Article
in English
| WPRIM
| ID: wpr-344906
ABSTRACT
<p><b>BACKGROUND</b>Hepatitis C virus (HCV) core antigen assays have been produced to exclude infectious donations collected during the preseroconversion window phase (PWP). For the same purpose, we evaluated the specificity and sensitivity of a novel hepatitis C virus NS3 antigen detection immunoassay and the application of this assay in clinical diagnosis.</p><p><b>METHODS</b>Samples from 77 healthy subjects, 173 anti-HCV positive patients and 3708 hepatitis patients other than HCV positive were tested with the HCV NS3 antigen assay. Some HCV NS3 antigen positive samples were further validated with HCV-RNA, neutralization and immunodot assays. Twenty-five sequential samples from 11 HCV NS3 antigen positive patients were subjected to kinetic study.</p><p><b>RESULTS</b>Only 48 (1.3%) of 3708 anti-HCV negative samples were positive for HCV NS3 antigen. Among them, 44 of 3030 samples from patients only infected with HBV were HCV NS3 antigen positive, 4 of the 445 samples from patients infected with other type hepatitis were HCV NS3 antigen positive. In addition, 42 (24.3%) of 173 anti-HCV positive samples were HCV NS3 antigen positive and all 77 samples from healthy subjects were negative to HCV NS3 antigen assay. Of the 15 HCV NS3 antigen positive samples, 9 (60%) were HCV-RNA positive. The neutralization and positive percentage of immunodot assay for 23 HCV NS3 antigen positive sera were 87.0% (20/23) and 69.6% (16/23) respectively. Of the 25 sequential samples from 11 HCV NS3 antigen positive patients, there was a negative correlation between the OD values and the duration of test (r = -0.989, P < 0.05), and there were correlations among their HCV NS3 antigen, HCV-RNA and anti-HCV titres. The anti-HCV antibodies of two sera were detected while their OD values of HCV NS3 antigen decreased gradually.</p><p><b>CONCLUSIONS</b>The HCV NS3 antigen detection assay showed perfect specificity and high sensitivity. Thus, it would be useful and economical as a routine test in laboratories for early diagnosis of HCV infection and prevention.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
RNA, Viral
/
Enzyme-Linked Immunosorbent Assay
/
Sensitivity and Specificity
/
Viral Nonstructural Proteins
/
Alanine Transaminase
/
Methods
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2007
Type:
Article
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