The expression and significance of hypoxia-inducible factor-1 alpha and related genes in abdominal aorta aneurysm / 中华外科杂志
Chinese Journal of Surgery
;
(12): 1509-1512, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-345054
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of hypoxia-inducible factor (HIF)-1alpha and related genes in abdominal aorta aneurysm (AAA) and explore the underlying pathogenesis.</p><p><b>METHODS</b>Twenty-two AAA specimens were collected and 5 normal abdominal aorta tissue were used as control. Northern blot, western blot and immunohistochemistry were used to evaluated the expression of HIF-1alpha mRNA and protein product. Western blot and immunohistochemistry method were also used to determine the expression of vascular endothelial growth factor (VEGF) and caspase-3. Microvessel density (MVD) was studied by immunohistochemistry stain of CD34.</p><p><b>RESULTS</b>The expression of HIF-1alpha mRNA and protein product were significantly higher in AAA than that in normal abdominal aorta (P < 0.01). The expression of VEGF and caspase-3 were also higher in AAA and both had a significantly positive relationship with HIF-1alpha expression (P < 0.01). Most of the positive cells located in VSMC and adventitia of AAA. The MVD counts were higher in AAA.</p><p><b>CONCLUSION</b>HIF-1alpha may have an important role the development of AAA, which maybe obtained by regulating the expression of VEGF or caspase-3.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Transcription Factors
/
RNA, Messenger
/
Immunohistochemistry
/
Aortic Aneurysm, Abdominal
/
Caspases
/
Vascular Endothelial Growth Factor A
/
Hypoxia-Inducible Factor 1, alpha Subunit
/
Caspase 3
/
Genetics
Limits:
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2004
Type:
Article
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