Serological and molecular study of three cases with a rare Bx02 blood group / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To determine the genotypes of three blood samples suspected as B subtype through DNA sequencing.</p><p><b>METHODS</b>The samples were first genotyped with PCR-SSP. Exons 6 and 7 of the ABO genes were subjected to PCR, direct sequencing, and cloned sequencing to determine the genotypes.</p><p><b>RESULTS</b>Serological results of the three samples were similar, with red cells being weakly agglutinatable by anti-B and serum containing anti-B. The samples were preliminarily genotyped as B/O1. Sequencing analysis showed that all three samples contained an O allele and a 905A>G mutation of the B gene, which was previously defined as Bx02.</p><p><b>CONCLUSION</b>Through sequencing analysis, the three samples typed as B subtype with serological testing were identified as Bx phenotype. The genotype of samples 1 and 2 was Bx02/O101, and that of sample 3 was Bx02/O102.</p>