Establishment of a screening method for AZF microdeletions by capillary technology and a clinical trial / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 550-554, 2016.
Article
in Zh
| WPRIM
| ID: wpr-345411
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish an accurate, fast and simple screening method for AZF microdeletions using capillary technology and use it for clinical testing.</p><p><b>METHODS</b>For each pair of primers, the 5' end of either forward or reverse primer was labeled with a FAM, JOE or TAMRA fluorescence dyes to establish multiplex quantitative fluorescence PCR systems for the establishment of a screening method of Y chromosome AZF microdeletions by capillary technology. The detection of Y chromosome AZF microdeletion was carried out on 725 cases of non-obstructive azoospermia, oligospermia or asthenospermia.</p><p><b>RESULTS</b>A screening method for Y chromosome AZF microdeletions using capillary technology was established. Thirty eight cases of AZF microdeletions were found among 725 cases of non-obstructive azoospermia, oligospermia or asthenospermia, which gave a deletion rate of 5.24%. Y chromosomal microdeletions were found in 8.62% of the azoospermia group, 6.75% of the oligozoospermic group, and 2.23% of the asthenospermia group.</p><p><b>CONCLUSION</b>An accurate, fast and simple screening method of Y chromosome AZF microdeletions by capillary technology has been established, which may have an important clinical value.</p>
Full text:
1
Index:
WPRIM
Main subject:
Sex Chromosome Aberrations
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Capillary Action
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Chromosome Deletion
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Chromosomes, Human, Y
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Diagnosis
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Azoospermia
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Sex Chromosome Disorders of Sex Development
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Multiplex Polymerase Chain Reaction
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Genetics
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Infertility, Male
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Adult
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2016
Type:
Article