Construction and characterization of an epitope-mutated Asia 1 type foot-and-mouth disease virus / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 96-104, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-345523
ABSTRACT
To generate an epitope-mutated foot-and-mouth disease virus (FMDV) as a marker vaccine, the infectious clone pAsia 1-FMDV containing the complete genomic cDNA of Asia 1 type FMDV was used as backbone, the residues at positions 27 and 31 in the 3D gene were mutated (H27Y and N31R). The resulting plasmid pAsia 1-FMDV-3DM encoding a mutated epitope was transfected into BHK-21 cells and the recombinant virus rAsia 1-3DM was rescued. The recombinant virus showed similar biological characteristics comparable with the parental virus. In serological neutralization test the antisera against recombine virus have a good reactivity with parental virus. The antisera against the mutant virus were shown to be reactive with the mutated epitope but not the wild-type one. The results indicated that the two virus strains could be distinguished by western blotting using synthetic peptides. This epitope-mutated FMDV strain will be evaluated as a potential marker vaccine against FMDV infections.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Neutralization Tests
/
Transfection
/
Cell Line
/
DNA, Complementary
/
Foot-and-Mouth Disease Virus
/
Genetics
/
Immune Sera
/
Epitopes
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2015
Type:
Article
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