Gene expression of AAV-ITR ssDNA mini vector in skeletal muscle of mice / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1720-1732, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-345551
ABSTRACT
AAV-ITR single strand DNA mini vector (AAV-ITR ssDNA mini vector) is a novel gene expression vector based on AAV-ITR. We have shown efficient gene expression of AAV-ITR ssDNA mini vector in HEK 293T. Here, we studied the efficacy of gene expression of AAV-ITR ssDNA mini vector in vivo. We injected the skeletal muscle of ICR mice separately with equal molars of AAV-ITR ssDNA mini vector, ITR mutated AAV-ITR single strand DNA mini vector (AAV-ITRmm ssDNA mutant vector), AAV-ITR dsDNA and pUC57-minivector-GFP, combined with TurboFect. Florescence microscope analysis of skeletal muscle section shows that AAV-ITR ssDNA mini vector had higher expression efficiency and longer expression period. We extracted DNA from the muscle three months after injection and quantified three vectors by Real-time PCR. RT-PCR analysis shows that there were highest copy numbers of AAV-ITR ssDNA mini vector existing in muscle. Stable existing of AAV- TR ssDNA mini vector in muscle could be the molecular basis of long term gene expression of the vector. The results suggest that AAV-ITR ssDNA mini vector might be a promising vector for gene therapy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA, Single-Stranded
/
Genetic Therapy
/
Gene Expression
/
Dependovirus
/
Muscle, Skeletal
/
Real-Time Polymerase Chain Reaction
/
Genetic Vectors
/
Genetics
/
Metabolism
/
Mice, Inbred ICR
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2014
Type:
Article
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