Immunogenicity of recombinant Lactobacillus casei expressing VP2 protein of infectious bursal disease virus in chickens / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1679-1690, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-345555
ABSTRACT
In order to determine immunogenicity and protective effect in chickens, we used the IBDV (Infectious bursal disease virus)-Vp2/Lactobacillus casei as antigen transfer system. First, the immunized and control chickens were challenged by IBDV/DQ at lethal dose to determine the protective ratio. Second, chickens were orallyand intranasally vaccinated twice with 10(9) CFU/mL pLA-VP2/L. casei, pLA/L. casei and PBS as negativecontrol and commercial vaccine as positive control. The bursa injury and the lesion score wererecorded post challenge. The level of specific IgG and sIgA in pLA-VP2/L. casei and positive control groups was significantly higher than that in negativecontrol groups. The protection efficacy in pLA-VP2/L. casei oral group was higher than that inintranasal group. The SI. of pLA-VP2/L. casei oral group was significant higher than other groups. The lesion score indicated the pLA-VP2/L. casei was safer than commercial vaccine for bursa. Collectively, the pLA-VP2/L. casei could be a vaccine candidate for IBDV.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Poultry Diseases
/
Blood
/
Recombinant Proteins
/
Viral Vaccines
/
Viral Structural Proteins
/
Chickens
/
Birnaviridae Infections
/
Infectious bursal disease virus
/
Allergy and Immunology
/
Lacticaseibacillus casei
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2014
Type:
Article
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