Preparation and vitality detection of protoplast in Salvia miltiorrhiza Bunge / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1612-1621, 2014.
Article
in Zh
| WPRIM
| ID: wpr-345562
Responsible library:
WPRO
ABSTRACT
We prepared protoplasts from Salvia miltiorrhiza Bunge suspension culture cells. Then, the protoplasts' vitality and functions were tested by fluorescein diacetate staining method and Fluo-3/AM flourescent probe. The optimal condition of protoplast isolation was Cellulase R-10 1.5%, Pectinase Y-23 0.3%, Macerozyme R-10 0.5%, 40 r/min 12 h, 600 r/min 5 min, and the protoplasts yield was 1.1x10(6) cells/g FW, the vitality was more than 95% by using fluorescein diacetate staining method. It has been confirmed that calcium fluorescent probe Fluo-3/AM can be successfully loaded into protoplasts.
Full text:
1
Index:
WPRIM
Main subject:
Protoplasts
/
Xanthenes
/
Cellulase
/
Chemistry
/
Cell Culture Techniques
/
Salvia miltiorrhiza
/
Fluorescent Dyes
/
Aniline Compounds
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2014
Type:
Article