Expression of limulus Factor C in silkworm larvae by Bac-to-Bac/BmNPV baculovirus expression system / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1594-1601, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-345564
ABSTRACT
Limulus Factor C, a serine protease zymogen from the amoebocytes of the limulus, has high affinity for endotoxin. When Factor C is activated by endotoxin, it hydrolyses artificial tripeptide substrate and measurable products are released, so it can be used as an alternative reagent for endotoxin analysis. Factor C gene of Tachypleus tridentatus was obtained through RT-PCR and the recombinant protein was expressed by Bac-to-Bac/BmNPV baculovirus expression system in silkworm larvae. The activity of Factor C was detected with diluted serum of silkworm larvae, and the sensitivity of endotoxin detected was 0.2 EU/mL when the serum was diluted at 1500. The silkworm larvae expressed limulus Factor C could be used to develop a new low-cost endotoxin test reagent.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Bombyx
/
Recombinant Proteins
/
Serine Endopeptidases
/
Baculoviridae
/
Enzyme Precursors
/
Arthropod Proteins
/
Genetic Vectors
/
Larva
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2014
Type:
Article
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