Optimization of the fermentation conditions for 5-keto-D-gluconic acid production / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1486-1490, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-345575
ABSTRACT
Gluconobacter oxydans converts glucose to gluconic acid and subsequently to 5-keto-D-gluconic acid (5-KGA), a precursor of industrially important L(+)-tartaric acid. To increase the yield of 5-KGA, fermentation conditions of 5-KGA production was optimized. Under the optimum medium and culture conditions in the shake flask, the highest 5-KGA production reached 19.7 g/L, increased by 43.8% after optimization. In a 5-L bioreactor, the pH was controlled at 5.5 and dissolved oxygen (DO) at 15%, 5-KGA production reached 46.0 g/L, raised at least 1.3 times than in the shake flask. Glucose feeding fermentation process was further developed, and the highest 5-KGA production of 75.5 g/L with 70% of yield was obtained, 32.0% higher than the highest reported value. Therefore, this newly developed fermentation process provided a practical and effective alternative for the commercial production of 5-KGA, and further of L(+)-tartaric acid.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tartrates
/
Industrial Microbiology
/
Bioreactors
/
Gluconobacter oxydans
/
Fermentation
/
Gluconates
/
Glucose
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2014
Type:
Article
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