Effects of overexpression of NADH kinase gene on ethanol fermentation by Saccharomyces cerevisiae / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1381-1389, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-345586
ABSTRACT
Glycerol is the main byproduct in ethanol production by Saccharomyces cerevisiae. In order to improve ethanol yield and the substrate conversion, a cassette about 4.5 kb for gene homologous recombination, gpd2ΔPGK1(PT)-POS5-HyBR, was constructed and transformed into the haploid strain S. cerevisiae S1 (MATa) to replace the GPD2 gene by POS5 gene. The NADH kinase gene POS5 was successfully over expressed in the recombinant strain S. cerevisiae S3. Comparing with the parent strain, the recombinant strain S. cerevisiae S3 exhibited an 8% increase in ethanol production and a 33.64% decrease in glycerol production in the conical flask fermentation with an initiatory glucose concentration of 150 g/L. Overexpression of NADH kinase gene seems effective in reducing glycerol production and increasing ethanol yield.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Saccharomyces cerevisiae
/
Industrial Microbiology
/
Chemistry
/
Phosphotransferases (Alcohol Group Acceptor)
/
Saccharomyces cerevisiae Proteins
/
Mitochondrial Proteins
/
Ethanol
/
Fermentation
/
Genetics
/
Glycerol
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2014
Type:
Article
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