Production of β-carotene by metabolically engineered Saccharomyces cerevisiae / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1204-1216, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-345604
ABSTRACT
β-carotene has a wide range of application in food, pharmaceutical and cosmetic industries. For microbial production of β-carotene in Saccharomyces cerevisiae, the supply of geranylgeranyl diphosphate (GGPP) was firstly increased in S. cerevisiae BY4742 to obtain strain BY4742-T2 through over-expressing truncated 3-hydroxy-3-methylglutaryl-CoA reductase (tHMGR), which is the major rate-limiting enzyme in the mevalonate (MVA) pathway, and GGPP synthase (GGPS), which is a key enzyme in the diterpenoid synthetic pathway. The β-carotene synthetic genes of Pantoea agglomerans and Xanthophyllomyces dendrorhous were further integrated into strain BY4742-T2 for comparing β-carotene production. Over-expression of tHMGR and GGPS genes led to 26.0-fold increase of β-carotene production. In addition, genes from X. dendrorhous was more efficient than those from P. agglomerans for β-carotene production in S. cerevisiae. Strain BW02 was obtained which produced 1.56 mg/g (dry cell weight) β-carotene, which could be used further for constructing cell factories for β-carotene production.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polyisoprenyl Phosphates
/
Saccharomyces cerevisiae
/
Basidiomycota
/
Beta Carotene
/
Farnesyltranstransferase
/
Metabolic Engineering
/
Genetics
/
Hydroxymethylglutaryl CoA Reductases
/
Metabolism
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2014
Type:
Article
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