Evaluation of the iNtRON VRE vanA/vanB Real-Time PCR Assay for Detection of Vancomycin-Resistant Enterococci
Annals of Laboratory Medicine
;
: 76-81, 2015.
Article
in English
| WPRIM
| ID: wpr-34572
ABSTRACT
BACKGROUND:
Recently, the iNtRON VRE vanA/vanB real-time PCR (iNtRON; iNtRON Biotechnology, Korea) assay, a multiplex real-time PCR method, was introduced. In this prospective study, we compared the iNtRON assay with the Seeplex VRE ACE detection kit (Seeplex; Seegene, Korea), a conventional multiplex PCR assay.METHODS:
A chromogenic agar-based culture, in which pre-selected vancomycin-resistant enterococci (VRE) was grown and subsequently plated on blood agar with vancomycin disks, was regarded as the reference method. A total of 304 consecutive rectal swab specimens were tested for VRE by culture and by iNtRON and Seeplex PCR assays. For the PCR assays, specimens were enriched for 16-24 hr before PCR.RESULTS:
VRE were isolated from 44 (14.5%) specimens by chromogenic agar-based culture. The clinical sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the iNtRON assay were 100% (95% confidence interval 89.8%-100%), 99.2% (96.9%-99.9%), 95.6% (83.6%-99.2%), and 100% (98.2%-100%), respectively, while those of the Seeplex assay were 97.7% (86.2%-99.9%), 99.6% (97.5%-99.9%), 97.7% (86.2%-99.9%), and 99.6% (97.5%-99.9%), respectively. The iNtRON assay had a detection limit of 3,159 copies/microL and 13,702 copies/microL for the vanA and vanB genes, respectively. No cross-reactivity was observed in 11 non-VRE bacterial culture isolates.CONCLUSIONS:
The overall performance of the iNtRON assay was comparable to that of a chromogenic agar-based culture method for prompt identification of VRE-colonized patients in hospitals. This assay could be an alternative or supportive method for the effective control of nosocomial VRE infection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reagent Kits, Diagnostic
/
Bacterial Proteins
/
DNA, Bacterial
/
Bacterial Typing Techniques
/
Gram-Positive Bacterial Infections
/
Carbon-Oxygen Ligases
/
Vancomycin Resistance
/
Real-Time Polymerase Chain Reaction
/
Vancomycin-Resistant Enterococci
Type of study:
Diagnostic study
/
Observational study
Limits:
Humans
Language:
English
Journal:
Annals of Laboratory Medicine
Year:
2015
Type:
Article
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