Effect of neferine on hepatic stellate cells in collagen-I, TIMP-1 and MMP-2 / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2206-2209, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-346413
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of neferine on Collagen-I, TIMP-1 and MMP-2 expressions and protein secretion of hepatic stellate cells.</p><p><b>METHOD</b>The hepatic stellate cell line HSC-T6 was cultured in vitro, and then randomly divided into 5 groups the control group, the platelet-derived growth factor (PDGF) group and PDGF + neferine (2, 6, 10 micromol x L(-1)) groups. All of the groups were cultured for 48 h, and their cells were collected to extract mRNA and detect Collagen-I, TIMP-1 and MMP-2 expressions with RT-PCR. Their cell supernatants were also collected to determine the protein content of three factors with ELISA.</p><p><b>RESULT</b>Compared with the control group, PDGF could remarkably increase the Collagen-I, TIMP-1 and MMP-2 expressions and protein secretion of hepatic stellate cells. Compared with the PDGF group, PDGF + neferine (6, 10 micromol x L(-1)) groups showed a notable decrease in the Collagen-I and mRNA expression and protein secretion along with the increase in the concentration, whereas the PDGF + neferine (2 micromol x L(-1)) group showed no significant change in the Collagen-I and mRNA expression and protein secretion. Compared with the PDGF group, three PDGF + neferine groups showed no notable change in MMP-2 expression and protein secretion.</p><p><b>CONCLUSION</b>Neferine can inhibit the Collagen-I, TIMP-1 and mRNA protein expression and protein secretion of PDGF-induced HSCs along with the increase in the concentration, but with not remarkable effect on the MMP-2 expression and secretion.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Drugs, Chinese Herbal
/
Cells, Cultured
/
Chemistry
/
Tissue Inhibitor of Metalloproteinase-1
/
Matrix Metalloproteinase 2
/
Collagen Type I
/
Benzylisoquinolines
/
Hepatic Stellate Cells
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article
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