Preparation and in vitro evaluation of curcumin CTPP-PEG-PCL micelles / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2109-2113, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-346432
ABSTRACT
To synthetize 3-carboxypropyl-triphenylphosponium bromide-polycaprolacton-CTPP-PEG-PC, and prepare curcumin CTPP-PEG-PCL micelles by using the self-assembled emulsion solvent evaporation method, in order to determine the critical micelle concentration (CMC) with the pyrene fluorescent probe technology, detect the particle size, entrapment efficiency (%), morpheme and in vitro release rate, and evaluate the cytotoxicity of hepatic stellate cells with MTT assay. The structure of CTPP-PEG-PCL had been identified by 1H-NMR spectra. Specifically, the CMC of polymer was 2.25 mg x L(-1), the average size was 190 nm, the drug content was (0.66 +/- 0.008) g x L(-1), and the entrapment efficiency was (94 +/- 0.6)%. The in vitro release results showed curcumin micelles had a significant higher inhibition ratio in the growth of hepatic stellate cells than crude curcumin (P < 0.05). This suggested that CTPP-PEG-PCL micelles feature low CMC, high encapsulation efficiency and notable inhibition effect in growth of hepatic stellate cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Polyesters
/
Polyethylene Glycols
/
Cell Line
/
Chemistry
/
Curcumin
/
Cell Proliferation
/
Hepatic Stellate Cells
/
Micelles
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article
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