Detection of tobacco mosaic virus (TMV) in Rehmannia glutinosa f. hueichingensis by IC-RT-PCR / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2092-2095, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-346436
ABSTRACT
<p><b>OBJECTIVE</b>To establish a rapid, sensitive and efficient detection method for tobacco mosaic virus (TMV), and provide technical support of TMV detection of Rehmannia glutinosa f. hueichingensis. The virus-free plantlets could be produced on a large scale to ameliorate breed degeneration caused by viral disease.</p><p><b>METHOD</b>Specific primers were designed based on the conserved region of coat protein(CP) gene of TMV. Immunocapture RT-PCR (IC-RT-PCR) was employed to detect TMV and the sequence of the products was detected.</p><p><b>RESULT</b>The expected nucleotide acid fragments were amplified by IC-RT-PCR. The homology of nucleotide acid sequence and amino acid sequence were 95.29% and 96.7% between the PCR products and the CP gene of TMV (accession number AY555269).</p><p><b>CONCLUSION</b>The method was established for the detection of TMV in R. glutinosa f. hueichingensis by IC-RT-PCR. This detection combined molecular biology technology with immunology, was convenient for a quick, sensitive and simple detection of TMV.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tobacco Mosaic Virus
/
Virology
/
Molecular Sequence Data
/
Base Sequence
/
Amino Acid Sequence
/
Reverse Transcriptase Polymerase Chain Reaction
/
Rehmannia
/
Allergy and Immunology
/
Genetics
/
Methods
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article
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