Preparation of ferulic acid, senkyunolide I and senkyunolide H from Ligusticum chuanxiong by preparative HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1947-1950, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-346465
ABSTRACT
Preparative HPLC was used to prepare ferulic acid, senkyunolide I and senkyunolide H from Ligusticum chuanxiong. The separation was conducted on a Shim-Pack Prep-ODS (20.0 mm x 250 mm, 5 microm) column with the mobile phase of methanol-0.2% glacial acetic acid (5050)at the flow rate of 5 mL x min(-1). The detection wavelength was 278 nm, and the purity of each compound was detected by HPLC analysis. Spectral data analyses including UV, ESI-MS and NMR were used to identify their structures. This method is simple, fast, which is suitable for preparation of standard reference of ferulic acid, senkyunolide I and senkyunolide H from L. chuanxiong and can meet the requirement of new drug research and development.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Benzofurans
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Coumaric Acids
/
Ligusticum
/
Methods
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article
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