Study on rapid screening and identifying hepatotoxic compounds in toosendan fructus / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1820-1822, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-346492
ABSTRACT
Fluorescein diacetate-labeled HepG2 cells model and flouresence automatic microscopy screening assay were used for fast screening 23 components from Toosendan Fructus, in which 5 components showed significant toxicity on HepG2 cells. The 10 compounds in the 2 components were tentatively identified with LC-MS(n), and 3 of them (meliasenin B, trichilinin D and 1-O-tigloy-1-O-debenzoylohchinal) were prepared and identified. Further experiments showed that the 3 compounds displayed dose-dependent toxicity on HepG2 cells, suggesting that these compounds in Toosendan Fructus may cause hepatotoxicity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Drugs, Chinese Herbal
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Melia
/
Drug-Related Side Effects and Adverse Reactions
/
Toxicity
/
Hep G2 Cells
/
Fruit
/
Liver
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article
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